Calculation of antibody and antigen concentrations from ELISA data using a graphical method.
نویسندگان
چکیده
A graphical method for determining the concentration of either the antibody or the antigen from ELISA data is presented in the form of a GWBASIC program. In the program, ELISAEQ, optical densities (OD) obtained from a 96-well ELISA plate can be input either directly by interfacing a microplate reader to the computer or manually. The program uses standard sample data, and selects the semilogarithmic linear range. Over this range, a least-squares method is used to determine the concentrations of interest. In addition, a hyperbolic interpolation formula is derived over the entire range for estimating the antibody or antigen concentration of the unknown samples whose OD is beyond the linear range.
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عنوان ژورنال:
- Journal of immunological methods
دوره 153 1-2 شماره
صفحات -
تاریخ انتشار 1992